Optimisation of CHO media and application in the biopharmaceutical industry
Cui • 2023 • SUNY-ESF Thesis
MOLECULAR MONITORING • BIOPROCESSES
From fluorescence to Raman, a more complete molecular view.
A-TEM combines absorbance, transmittance and excitation–emission matrices. PoliSpectra adds the vibrational specificity of Raman in automated plaque screening. Together, they accelerate decisions in development, characterization and quality control.
No markingMinimum preparationChemometric models
HORIBA SOLUTIONSMolecular monitoring
A-TEEM + RAMAN
A-TEEM
AQUALOG-NextComplex matrices
A-TEEM
DuettaResearch and Routine
A-TEEM
VelociBiopharma and PAT
RAMAN
PoliSpectra RPRPlate screening
AQUALOG-NextComplex matrices
A-TEEMDuettaResearch and Routine
A-TEEMVelociBiopharma and PAT
RAMANPoliSpectra RPRPlate screening
AbsorbanceFluorescence EEMRaman Signature
4complementary platforms
< 1 sEEM on Duetta, as shown
96 wellsPolySpectra default configuration
24Local technical and scientific PDFs
ANALYTICAL OBJECTIVE
Molecular information obtained
Select a need. The page highlights the most adherent analytical route — and when it is worth combining techniques.
ROAD RECOMMENDED
See solutions →
A-TEEM for characterization; Raman for complementary screening
Duetta or Veloci evaluate concentration, conformational changes and intrinsic signatures. PoliSpectra can expand the screening of formulations by vibrational signature.
PORTFOLIO HORIBA
Four platforms. A molecular ecosystem.
A-TEM • COMPLEX Sample matrices
HORIBA SCIENTIFIC
AQUALOG®-Next
Fast molecular digital printing for complex aqueous matrices, uniting EEM, absorbance and internal filter effect correction.
- Characterization of organic matter, contaminants and matrix changes
- Applies to research, method development and QC/QA
- A-TEM platform with EzSpec and multivariate analysis
Complex matricesEEMUV-Vis
A-TEM MaterialsPUBLICATIONS ASSOCIATED
AQUALOG in bioprocesses
Publications that directly cite AQUALOG/AQUALOG 800.
Algal proteins: impact of extraction techniques
Nunes et al. • 2026 • Food and Bioprocess Technology
A-TEM • FLEXIBLE BANK
HORIBA SCIENTIFIC
Duetta™
Simultaneous fluorescence and absorbance for molecular research, routine testing and development of methods in a single instrument.
- EEM in up to 1 second and A-TEM in up to 30 seconds, dependent on the sample
- Quantification of proteins in low concentration and A280
- DNA/RNA, concentration curves and pass/fail tests in EzSpec
ProteinsDNA/RNA250–1100 nm
Duetta MaterialsARTICLES WITH EQUIPMENT
Duetta in biological systems
The four articles below directly cite Duetta or Duetta-Bio.
Kombucha, silver nanoparticles and biofilm suppression
Opris et al. • 2026 • IJMS
Kombucha-mediated nanoparticles against Candida
Opris et al. • 2026 • CIMB
Brain organoids with fluorescent nanodots
Sprengel et al. • 2026 • RSC Advances
FRET and DNA polymerase assembly kinetics δ
Norris & Hedglin • 2024 • JBC
A-TEEM • BIOFARMA & PAT
HORIBA SCIENTIFIC
Veloci™ BioPharma Analyzer
A-TEM oriented to biopharmaceutical workflows, with multivariate models, traceability and integration to process environments.
- Batch-to-lot variability and crop media composition
- mAbs, AAV, vaccines, proteins and stability of formulations
- Direktor for OPC-UA modeling and interface for PAT integration
BiofarmaQuimiometriaOPC-UA
Veloci MaterialsIMPLEMENTATION STUDIES
Veloci for cell-culture media and biopharmaceuticals
Workflow A-TEM-specific materials developed for Veloci.
Qualitative and quantitative analysis of culture media
HORIBA • Veloci BioPharma Analyzer
Monitoring the variability of culture media
HORIBA • Veloci e A-TEEM
2D fluorescence in mAb purification monitoring
A-TEM • Bioprocess measurement and control
RAMAN • PLATE TRIAGE
HORIBA SCIENTIFIC
PoliSpectra® RPR
Automated Raman reader for non-destructive molecular screening on plates, designed to integrate high-throughput screening flows.
- Settings at 532 or 785 nm and standard 96 well plate
- NTPs, proteins and glucose demonstrated in a technical study HORIBA
- Automation, robot door, heating and control OPC-AU
RAMAN IN BIOPROCESSES
PoliSpectra and Raman applications
A specific technical study of RPR and three articles that support the related Raman workflows.
Rapid Raman Plate Reader in Biopharmaceutical Applications
HORIBA • NTPs, proteins, glucose and model particles
Raman in drug discovery and cellular response
Dunnington et al. • 2024 • Analytical Chemistry
Transfer learning in monitoring Bioprocess Raman
Hüpfl et al. • 2026 • BBSE
Raman time-gated to increase upstream sensitivity
Shaikat et al. • 2026 • BBSE
COMPLEMENTARY TECHNICALS
A-TEEM or Raman?
The best response depends on the molecule, matrix and decision that will be made.
CriteriaA-TEEMRadan plate
Analytical signalAbsorbance + fluorescence EEMMolecular vibrations
Key strengthHigh sensitivity to fluorophores and microenvironmentChemical specificity, no markers
Water and simple sugarsLow interference; simple sugar may not generate direct signalThey can be measured according to concentration and matrix
FormatoCubet/laboratory workflow and PAT according to platformAutomated microplate screening
Best combinationSensitivity characterization and identity/quality modelsOrthogonal confirmation and composition screening
ANALYTICAL STRATEGY
From screening to analytical assessment.
- 01Set the QAIdentity, concentration, stability or composition.
- 02Get fingerprintsA-TEM, Raman or both in representative samples.
- 03Build the modelPCA, PLS or orthogonal reference classification.
- 04Validate and transferPerformance, robustness and workflow integration.
TECHNICAL AND SCIENTIFIC BIBLIOTE
Select, preview and download.
The collection brings together 15 manufacturer materials and 9 academic PDF publications, all stored locally.
0 selected
How to read the evidence: “direct use” means that the publication cites AQUALOG, Duetta or Duetta-Bio. The Raman articles are identified as “related evidence of the technique”: they support applications of Raman in bioprocesses, but were not performed in the PolySpectra RPR. The specific results of NTPs, proteins and glucose in RPR remain technical data from HORIBA.
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Inform the bioprocess, analytes, expected range and frequency of analysis. SENS structures the proof of concept and technical configuration.